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KMID : 0358419740170120729
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Korean Journal of Obstetrics and Gynecology
1974 Volume.17 No. 12 p.729 ~ p.734
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Studies on the Amniotic Fluid by Paper Electrophoresis and Agar-gel Diffusion
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Abstract
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The origin and formation of amniotic fluid have been the subject of many scientific investigations. More than 20 different theories have been proposed in attempts to explain the formation of the amniotic fluid but only few have survived the test of time.
Although the mechanical function and physiochemical view of the amniotic fluid has been known well, the immunological and biochemical function of amniotic fluid are obscure at present time.
Electrophoretic techniques and antignic analysis have demonstrated the presence of serum proteins in umbilical cord, maternal serum and amniotic fluid during pregnancy and have stimulated investigations concerned with their origin and the factors which regulate their presence and concentration.
The evidence to date, from both in vivo and in vitro studies suggest that the paper electrophretic patterns of amniotic fluid proteins were similar to that of maternal serum.
Utilizing the techniques of paper electrophoreses and agar gel diffusion method, concentrations in amniotic fluid, metrnal and cord serum have been analyzed in the term pregnant women.
The results are summarized as follows;
1) The total amount of protein in the amniotic fluid is 263.8±16.5mg/dl compared with 7.3±0.2g/dl in the maternal serum and 6.5±0. 3g/dl in the umbilical cord serm.
2) The fractions of amniotic protein are similar to that of maternal serum by means of paper electrophoresis and the fraction of albumin in amniotic fluid is 57.0± 0.5 mg/dl.
3) By the agar-gel diffusion method, immunoglobulin G and M are detected in both amniotic fluid and fetal cord blood but lg A is not detected.
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